Rant:
I remember when we had to put on a condom onto a life-sized model in sex ed at school. That wasn't the worst part, though. Afterwards, our hands were covered in lube, so all of the boys in our class, including me, ran into the nearest toilets to wash our hands. One of the teachers was suspicious of us, and forced us out, so we couldn't wash our hands.
What? Someone blocked the words 'condom' and 'sex'?
For anybody still wondering where FTD has gone, here it is.
Tural wrote:Gametag told me it was and I never did a fact check.
I think he got it from the site you posted with screenshots and such.
$20 says he will now call me stupid for this, even though he stated it.
It said Tom's pictures and Scotty was in every one of them I saw, and he referred to them as himself in there. I think anyways, I dunno. Point is ur dum.
My rant is the phobia thread. I look at that, expecting it to be built on a discussion of {TP} Spartan's post in the happiness thread. I can't fucking tell, because I Ctrl-W'd my way out of there really fucking fast, considering that the first thing I see is the same god damn image, but in tags instead of a link. Way to fucking go, DrXThirst. Maybe someone is horribly afraid of spiders, and literally won't sleep because of that. Ugh.
(7:15:27 PM) Xenon7: I BRUK THE FIRST PAGE OMGOMGOMG RONALD REGAN
We injected a plasmid from a jellyfish into a colony of bacteria to make the bacteria glow under a black light, but for whatever reason the bacteria rejected the plasmid, so they died on the antibacterial plate (the plasmid would have made them impervious to the type of antibacterial solvent used on the plate). I was looking forward to seeing them glow, too :\ (We used toothless E.Coli (harmless)).
We injected a plasmid from a jellyfish into a colony of bacteria to make the bacteria glow under a black light, but for whatever reason the bacteria rejected the plasmid, so they died on the antibacterial plate (the plasmid would have made them impervious to the type of antibacterial solvent used on the plate). I was looking forward to seeing them glow, too :\ (We used toothless E.Coli (harmless)).
Dude, rofl. You can't just insert random pieces of DNA into other organisms and expect it to happily accept it (unless you use the viral method with transcriptase and such - but that would need professional lab equipment and money).
Last edited by reanimation-06 on Sat Feb 16, 2008 5:44 am, edited 1 time in total.
We injected a plasmid from a jellyfish into a colony of bacteria to make the bacteria glow under a black light, but for whatever reason the bacteria rejected the plasmid, so they died on the antibacterial plate (the plasmid would have made them impervious to the type of antibacterial solvent used on the plate). I was looking forward to seeing them glow, too :\ (We used toothless E.Coli (harmless)).
Dude, rofl. You can't just insert random pieces of DNA into other organisms and expect it to happily accept it (unless you use the viral method with transcriptase and such - but that would need professional lab equipment and money).
I didn't want to explain the process we used. Other group's got their bacteria to glow just fine. Basically, we just inserted millions of plasmids into a container with a colony of bacteria, and heated the bacteria up in water for exactly 50 seconds to stress them to take the plasmid (Bacteria experiments with plasmids when they need to). Then we let them sit in a tube with food and let them get used to the plasmid. After that we put them on the plate, and set them in an incubator.
We did a lot more than that, though. The whole thing took a week to do.
We injected a plasmid from a jellyfish into a colony of bacteria to make the bacteria glow under a black light, but for whatever reason the bacteria rejected the plasmid, so they died on the antibacterial plate (the plasmid would have made them impervious to the type of antibacterial solvent used on the plate). I was looking forward to seeing them glow, too :\ (We used toothless E.Coli (harmless)).
Dude, rofl. You can't just insert random pieces of DNA into other organisms and expect it to happily accept it (unless you use the viral method with transcriptase and such - but that would need professional lab equipment and money).
I didn't want to explain the process we used. Other group's got their bacteria to glow just fine. Basically, we just inserted millions of plasmids into a container with a colony of bacteria, and heated the bacteria up in water for exactly 50 seconds to stress them to take the plasmid (Bacteria experiments with plasmids when they need to). Then we let them sit in a tube with food and let them get used to the plasmid. After that we put them on the plate, and set them in an incubator.
We did a lot more than that, though. The whole thing took a week to do.
Oh... with the heating + the concentration of plasmids, it would have worked. Did you actually extract the plasmids?
What grade level is this?
I come home from my friends party to find out someone spilled *** water all over my *** guitar hero 3 guitar!!! WTF!!! Now my *** orange and green button's don't work for shit! ***!!
Tural, i Realize that was addressed but i thought i would add my own comment, there was no need for you to remark on my reply as it served no use at all.
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